Fig. 3.

Specificity of the m-PCR method. a: Lanes 1–7, seven virus templates were detected (102 bp for FAdV, 140 bp for DHAV, 172 bp for DEV, 288 bp for DTMUV, 330 bp for NDV, 435 bp for AIV, and 516 bp for NDPV); Lane 8, result of the m-PCR method (mixture of the same concentrations of seven viruses); M, DL600 marker; NC, negative control. b: Specificity of the m-PCR method with other pathogens; 1 (M), DL600 marker; 2 (+), positive control; 3 (NC), negative control; 4 (DRV), duck Reovirus; 5 (DHAV-3), DHAV serotype 3; 6 (APMV-4), Avian paramyxoviruses serotype 4; 7 (APMV-8), Avian paramyxoviruses serotype 8;8 (P. multocida), Pasteurella multocida;9 (C. perfringens), Clostridium perfringens; 10 (E. coli), Escherichia coli; 11 (RA), Riemerella anatipestifer; 12 (SE), Salmonella