Fig. 1.

Multiple sequence alignment of the DNA-binding and HR-A/B domains of wheat Hsfs. The protein sequence alignment was performed using the BioEdit software. a Multiple alignment clearly reveals that the DBD domains of the wheat Hsfs are highly conserved. The secondary structure elements of the DBD (α1-β1-β2-α2-α3-β3-β4) are shown above the alignment. b The scheme at the top shows the boundaries and locations of the HR-A, insert and HR-B regions within the HR-A/B domains. The structures between HR-A and HR-B consist of 21 and 7 amino acid insertions in the TaHsfAs and TaHsfCs, respectively