. 2019 Apr 2;16:41. doi: 10.1186/s12985-019-1148-2

Table 1.

Summary of PRV testing by real-time, reverse transcriptase PCR (RT-qPCR) for all samples in this study

Collection Date	Location¹	Fish ID	# on map²	Fish tissue	AVC #³	PRV Seg. L1 Cts	PRV Seg. S1 product⁴
Aug-27-2017	Home Port Bellingham-WA	WFC-2011-75	1	Gill	VT01192018–07	28.09	Positive
				Heart	VT01192018–08	31.74
				Head kidney	VT01192018–09	28.85
		WFC-2011-76	2	Gill	VT01192018–10	30.77
				Heart	VT01192018–11	32.11
				Head kidney	VT01192018–12	30.33
		WFC-2011-77	3	Gill	VT01192018–13	29.65
				Heart	VT01192018–14	30.52
				Head kidney	VT01192018–15	28.63
		WFC-2011-78	4	Gill	VT01192018–16	27.67	Positive
				Heart	VT01192018–17	30.96
				Head kidney	VT01192018–18	28.67
		WFC-2011-79	5	Gill	VT01192018–19	28.78
				Heart	VT01192018–20	27.72
				Head kidney	VT01192018–21	25.73	Positive
		WFC-2011-80	6	Gill	VT01192018–22	27.78
				Heart	VT01192018–23	26.52
				Head kidney	VT01192018–24	26.53
		WFC-2011-81	7	Gill	VT01192018–25	29.33
				Heart	VT01192018–26	26.94	Positive
				Head kidney	VT01192018–27	26.44
		WFC-2011-82	8	Gill	VT01192018–28	26.15
				Heart	VT01192018–29	26.98
				Head kidney	VT01192018–30	29.91
		WFC-2011-83	9	Gill	VT01192018–31	30.10
				Heart	VT01192018–32	25.94
				Head kidney	VT01192018–33	27.03	Positive
		WFC-2011-103	10	Gill	VT01192018–34	31.19
				Heart	VT01192018–35	29.53
				Head kidney	VT01192018–36	27.98
		WFC-2011-104	11	Gill	VT01192018–37	31.68
				Heart	VT01192018–38	28.91
				Head kidney	VT01192018–39	30.12
		WFC-2011-105	12	Gill	VT01192018–40	30.12
				Heart	VT01192018–41	29.99
				Head kidney	VT01192018–42	29.06
		WFC-2011-106	13	Gill	VT01192018–43	24.89	Positive
				Heart	VT01192018–44	26.39
				Head kidney	VT01192018–45	24.10
Sept-11-2017	Home Port Bellingham-WA	WFC-2011-107	14	Gill	VT01192018–46	24.68
				Heart	VT01192018–47	26.09
				Head kidney	VT01192018–48	23.72	Positive
		WFC-2011-108	15	Gill	VT01192018–49	25.28
				Heart	VT01192018–50	25.04
				Head kidney	VT01192018–51	23.27
		WFC-2011-109	16	Gill	VT01192018–52	23.60	Positive
				Heart	VT01192018–53	24.60	Positive
				Head kidney	VT01192018–54	27.32
		WFC-2011-110	17	Gill	VT01192018–55	28.42
				Heart	VT01192018–56	28.76
				Head kidney	VT01192018–57	26.38
Sept-14-2017	Lower Fraser River-BC	AE01	18	Gill, Heart	VT10042017–372	33.00	Negative
Oct-08-2017	Johnstone St-BC	AE02	19	Gill, Heart, Liver	VT10252017–405	27.33	Positive
Oct-10-2017	Lower Fraser River-BC	AE03	20	Gill, Heart, Liver	VT10252017–407	30.41	Positive
Oct-17-2017	Johnstone St-BC	AE04	21	Gill, Heart, Liver	VT10252017–408	32.04
	St. of Juan De Fuca-WA	WFC-2011-13	22	Gill	VT01192018–01	0
				Heart	VT01192018–02	35.9
				Head kidney	VT01192018–03	34.10
Nov-01-2017	Salish Sea-BC	AE05	23	Heart, Liver, Spleen, Head kidney	VT11022017–423	25.86	Positive
Dec-31-2017	Skagit River, Birdsview-WA	WFC-2011-14	24	Gill	VT01192018–04	35.60
				Heart	VT01192018–05	33.47
				Liver	VT01192018–06	34.74
Jan-25-2018	Skagit River, mile 46-WA	WFC-2011-111	25	Gill	VT04202018–118	29.52	Negative
				Heart	VT04202018–119	25.97	Positive
				Liver	VT04202018–120	29.64	Positive
				Eggs	VT04202018–121	31.10	Positive
Jan-24-2018	Skagit River, mile 46-WA	WFC-2011-112	26	Gill	VT04202018–122	32.91	Negative
				Heart	VT04202018–123	29.76	Positive
				Liver	VT04202018–124	29.80	Positive
				Eggs	VT04202018–125	34.78	Negative
Jan-26-2018	Skagit River, mile 46-WA	WFC-2011-113	27	Gill	VT04202018–126	31.24	Negative
				Heart	VT04202018–127	28.76	Negative
				Liver	VT04202018–128	30.66	Negative
Apr-2018	Retail “Product of Iceland”	WFC-2011-114	28	Muscle	VT04202018–129	24.94	Positive

¹Location source of samples included Home Port, Bellingham Bay, Washington (Home Port Bellingham-WA), Strait of Juan De Fuca, Port Townsend, Washington (Strait of Juan De Fuca-WA), Skagit River near Birdsview, Washington (Skagit River, Birdsview-WA), Skagit River at mile 46, Washington (Skagit River, mile 46-WA), Lower Fraser River, BC-Canada (Lower Fraser River-BC), Johnstone St., BC-Canada (Johnstone St-BC), Salish Sea, BC-Canada (Salish Sea-BC), and market-bought fish from a retail store in Redmond, Washington, labeled “Product of Iceland” (Retail “Product of Iceland”)

²# on map refers to the location source of the fish on the map shown as Fig. 1

³AVC # denotes accession number of sample at the testing laboratory at the Atlantic Veterinary College, University of Prince Edward Island

⁴Selected samples were tested in conventional RT-PCR for full-length PRV S1 segment. The presence of a 1100 base pair PCR product band in agarose gel is indicated as Positive . Negative denotes no band seen in agarose gel