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. 2019 Apr 1;18:109. doi: 10.1186/s12936-019-2748-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Showing the regression between rtPCR-based assays for DBS (C_t) and rtPCR-based assays for EPF (C_t) for the singleplex assay to detect generic Plasmodium (18S rDNA gene). The red fitted line shows the predicted value and cross line is the cut off 45 C_t. The blue dashed lines show the 95% prediction interval. The mean C_t value of EPF (C_t) is 36.3, and DBS (C_t) is 33.3, and the actual difference between DBS (C_t) and EPD (C_t) is 2.3 (95% Cl 1.1–3.5)