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. 2019 Apr 2;17:108. doi: 10.1186/s12967-019-1852-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of the ZCCHC13 subcellular localization and ZCCHC13-specific antibodies in human sera. a The endogenous ZCCHC13 protein was distributed in the nucleus of Huh7 cells. Merged images showed the co-localization between the ZCCHC13 protein and nuclei stained with DAPI. b The recombinant ZCCHC13 protein was expressed at the predicted molecular size of approximately 18 kD, as particularly evident in the lane for the 150 mM imidazole elution buffer. c Responses of serum from patients with HCC and healthy donors to ZCCHC13, as determined using an ELISA (****p < 0.0001)