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. 2019 Apr 2;17:108. doi: 10.1186/s12967-019-1852-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — 5-Aza-induced demethylation increases ZCCHC13 expression in human liver cancer cells. a Results of MSP, b RT-qPCR, c RT-PCR and d Western blot analysis showing that 5-Aza attenuated the methylation of the ZCCHC13 promoter in HepG2 cells. U: Unmethylated MSP amplicons; M: methylated MSP amplicons. GAPDH was used as an internal control. The results are presented as the mean ± SD of three independent experiments (*p < 0.05 and **p < 0.01)