Fig. 3.

Methodology for virus production and infection experiments. a Viruses are produced in HEK293T cells by co-transfection with a plasmid encoding the OGH reporter virus and a plasmid encoding the VSV-G envelope. LEDGINs are added to the cell medium. 72 h post transfection, viruses are harvested from the supernatant, concentrated and washed to remove remaining compound. These viruses can be used to infect different target cells. b Different target cells (SupT1, Jurkat, MT-4) were infected with the double reporter virus. Three days post infection (p.i.) samples were taken for flow cytometry and virus was washed away. Cells were reactivated with TNFα eight days p.i. and flow cytometry samples were taken 24 h after reactivation. TNFα Tumor Necrosis Factor alpha, VSV-G vesicular stomatitis virus G