Figure 6.
LTB4 release from aspirin-activated eosinophils. Eosinophils isolated from peripheral blood of control subjects (open bars: n = 12) and patients with asthma (solid bars: n = 11) were activated with lysine aspirin (LysASA; 10 mM), the combination of ionomycin (2 μg/ml) with low (L; 33 ng/ml) or high (H; 100 ng/ml) dose phorbol 12-myristate 13-acetate (PMA), trypsin 1 μM, or platelet-activating factor (PAF) 1 μM for 30 minutes. Supernatants were collected and LTB4 levels were quantified by enzyme immunoassay (pg/105 cells). Data are presented as mean ± SEM with *P < 0.001 or **P < 0.02 in comparison with unstimulated cells.