Figure 4.

Enzymatically active INPP4A is secreted in cell culture–conditioned media. (A) INPP4 activity, depicted by the amount of phosphatidyl inositol 3-phosphate (PI[3]P) produced, in the conditioned media of bronchial epithelial cells (BEAS-2B) as measured by competitive ELISA. Results are expressed as the mean ± SEM (n = 4). (B and C) Representative Western blot of INPP4A expression and secretion in the cell lysate and conditioned media of BEAS-2B cells (B) and primary normal human bronchial epithelial (NHBE) cells (C). Readers may view the uncut gels for B in the data supplement.