Fig. 4.
The expression of Ca. L. asiaticus effectors in Duncan (a), Washington navel (b), citron (c), Cleopatra (d), Pomeroy (e), and Carrizo (f) after 7-day ACP infestation in a detached leaf assay. Three randomly selected Ca. L. asiaticus positive leaves were used for RNA isolation and expression analysis. The Ct values of each effector generated by RT-qPCR were normalized with citrus endogenous control UPL7 and transformed into relative quantification (RQ) values (2-ΔCt). The effectors with expression detections were subject to pair-wise comparison of standard least-square means (LS means) with Student’s t-test (p < 0.05). Expression levels indicated with the same letter are not significantly different. Bars are means ± standard error (n = 3)