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. 2016 Nov 21;2:16063. doi: 10.1038/micronano.2016.63

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rapid assembly of multilayer PDMS microfluidic devices achieved with 3D-printed molding. (a) Use of a 3D-printed stamp to selectively apply uncured PDMS to non-channel areas. (b) Conceptual illustration of alignment and assembly of multilayer microfluidic device with illustration of intralayer fluid flow. (c) Intruded stamp topography. (d) Second layer of six-layer device with corresponding stamp, which exhibits extruded stamp topography. (e–h) Rapid assembly of multilayer microfluidic device: (e) CAD model, (f) stacked individual layers, (g) assembled and bonded, and (h) fluid flow spiraling between layers and mixing. 3D, three-dimensional; PDMS, poly(dimethylsiloxane).