Table 2.
Gene expression validation by RT-qPCR
| Gene symbol | Microarray fold change | RT-qPCR fold change | p-value |
|---|---|---|---|
| SPP1 | 6.9 | 7.12 ± 3.28 | 0.012 |
| MMP9 | 4.2 | 6.68 ± 2.95 | 0.019 |
| CTSS | 2.6 | 2.72 ± 0.90 | 0.041 |
| CD163 | 2.2 | 2.12 ± 0.48 | 0.028 |
| CCR7 | 2.06 | 2.71 ± 0.59 | 0.034 |
| SFRP4 | 1.91 | 2.19 ± 0.54 | 0.034 |
| DKK3 | 1.9 | 1.73 ± 0.24 | 0.010 |
| AQP3 | 1.9 | 2.36 ± 0.50 | 0.023 |
| CTSL1 | 1.6 | 1.62 ± 0.31 | 0.050 |
| CD44 | 1.5 | 1.35 ± 0.20 | 0.158 |
| NRF1 | 1 | 1.03 ± 0.08 | 0.875 |
| ATGL | 0.81 | 0.86 ± 0.08 | 0.084 |
| GAPDH | 0.81 | 1.11 ± 0.12 | 0.754 |
| AQP7 | 0.73 | 0.82 ± 0.06 | 0.008 |
| PLAT | 0.73 | 0.74 ± 0.09 | 0.019 |
| LEP | 0.73 | 0.84 ± 0.12 | 0.158 |
| CIDEC | 0.69 | 0.79 ± 0.09 | 0.028 |
| CD36 | 0.68 | 1.00 ± 0.08 | 0.638 |
| RARRES2 | 0.65 | 0.74 ± 0.06 | 0.003 |
| CES1 | 0.63 | 0.78 ± 0.12 | 0.028 |
| RBP4 | 0.54 | 0.88 ± 0.14 | 0.158 |
| SAA1 | 0.5 | 0.98 ± 0.19 | 0.480 |
| LHS | 0.38 | 0.83 ± 0.09 | 0.071 |
Changes in mRNA levels were determined using microarrays and RT-qPCR before (V1) and after 12 sessions of mechanical massage (V2). Data represent mean ± SEM (ratio of mRNA levels at V2 divided by values at V1) of 12 subjects.