Figure 4.

Changes in intestinal microbiota composition, following human or murine fecal microbiota transplantation in the multi-drug resistant P. aeruginosa infected mice. Secondary abiotic mice were challenged with multi-drug resistant P. aeruginosa on day (d) 0 and d1 by gavage and subjected to peroral fecal microbiota transplantation (FMT) from either human or murine donors at d7, d8, and d9 postinfection (p.i.; i.e., D0, D+1, and D+2) or to PBS (mock). Seven days later (i.e., D+7), the fecal commensal microbiota composition was assessed applying culture-independent 16S rRNA methods quantitating the total eubacterial load and main commensal bacterial groups such as enterobacteria, enterococci, lactobacilli, bifidobacteria, Bacteroides/Prevotella species, Clostridium coccoides group, Clostridium leptum group, and Mouse Intestinal Bacteroides (expressed as gene copies per ng DNA). Box plots represent the 75th and 25th percentiles of medians (black bar inside the boxes). The total range, significance levels (p-values) determined by the Mann–Whitney U, and total numbers of analyzed mice are indicated. Data were pooled from 4 independent experiments