Fig. 5.
Subcellular localization after cotransfection HEK293 cells were transiently co-transfected with expression plasmids encoding MC3R-YFP or MC4R-YFP and GHSR, NPY2R, µ-OPR or MC4R, GPR7, 5HTR1B, CB1R coupled to CFP. Cells were imaged by confocal laser scanning microscopy 24 h after transfection. CFP-tagged receptors were visualized by excitation at 458 nm, whereas YFP-tagged GPCRs were excitated at 488 nm. Emission wavelengths were detected by using a LP505 filter. Representative cells are shown.