Fig. 2.

PTEN is under-expressed in CRC cell lines through mir-26a regulation. a Expression of PTEN and miR-26a in CRL1790 (control), HCT116, SW480 and SW620 (CRC cell lines) normalized with GAPDH or RNU6, respectively. b Western blot was performed to detect the expression of PTEN in the different cell lines and a mouse healthy tissue (H.T), b-actin was used as loading control. c mir-26a was transfected in HCT116 cell line and was measured by real-time, mir-26a expression was normalized with RNU6 PCR. d Luciferase reporter assay using mir-26a/PTEN interaction region in HCT116 cell lines normalized with empty vector