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. Author manuscript; available in PMC: 2019 Apr 2.
Published in final edited form as: Dev Biol. 2018 May 25;441(2):319–327. doi: 10.1016/j.ydbio.2018.05.009

Fig. 2. Pre-treatment with an anxiolytic drug, buspirone, reduces bottom dwelling behavior in surface and cavefish alike.

Fig. 2.

(A) Diagram of experimental workflow. (B) Representative trajectories of a surface and Pachón adult in a novel tank before and after drug treatment. (C) Quantifications of total duration spent exploring the top half of the novel tank in 10-min trials pre- and post-treatment. Striped bars - Surface(−) vs. Surface(+): P = 0.019; Pachón(−) vs. Pachón(+): P = 0.0074. (D) Quantification of total duration that individuals were immobile. Striped bars - Pachón(−) vs. Pachón(+): P = 0.010. Surface(−) vs. Pachón(−): P = 0.0069. (Surface without buspirone treatment: n = 5; Surface with buspirone treatment: n = 4; Pachón without buspirone treatment: n = 5; Pachón with buspirone treatment: n = 5) Darker-colored bars denote baseline measurements, while lighter-colored bars denote second trials. (−) indicates system water, (+) indicates 12.5 mg/L buspirone dissolved in system water. Striped bars indicate sets of groups that undergo buspirone treatment, whereas solid bars indicate groups that were treated with system water only. Data analyzed by paired t-test: * indicates P < 0.05; ns indicates not significant. Error bars ± s.e.m.