KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat Polyclonal anti-Pum1 | Bethyl Laboratories | Cat#A300–201A; RRID: AB_2253218 |
| Rabbit Polyclonal anti-Pum2 | Bethyl Laboratories | Cat#A300–202A; RRID: AB_2173752 |
| Rabbit monoclonal anti-Pum1 | Abcam | Cat#ab92545; RRID: AB_10563695 |
| Rabbit monoclonal anti-p27 KIP 1 | Abcam | Cat#92741; RRID: AB_10561762 |
| Mouse monoclonal anti-Actin | Sigma-Aldrich | Cat#A1978; RRID: AB_476692 |
| Mouse monoclonal anti-α Tubulin | Santa Cruz Biotechnology | Cat#sc-8035; RRID: AB_628408 |
| Rabbit monoclonal anti-GAPDH (D16H11) | Cell Signaling Technology | Cat#5174; RRID: AB_10622025 |
| Rabbit Polyclonal anti-Cleaved Caspase-9 | Cell Signaling Technology | Cat#9505; RRID: AB_2290727 |
| Rabbit monoclonal anti-Cleaved Caspase-3 | Cell Signaling Technology | Cat#9664; RRID: AB_2070042 |
| Rabbit monoclonal anti-Phospho-Histone H3 (Ser10) (D2C8) | Cell Signaling Technology | Cat#3377; RRID: AB_1549592 |
| Mouse monoclonal anti-cdc2 (POH1) | Cell Signaling Technology | Cat#9116; RRID: AB_2074795 |
| Rabbit monoclonal anti-CDK2 (78B2) | Cell Signaling Technology | Cat#2546; RRID: AB_2276129 |
| Mouse monoclonal anti-CDK4 (DCS156) | Cell Signaling Technology | Cat#2906; RRID: AB_2078399 |
| Mouse monoclonal anti-CDK6 (DCS83) | Cell Signaling Technology | Cat#3136; RRID: AB_2229289 |
| Mouse monoclonal anti-Cyclin D1 (DCS6) | Cell Signaling Technology | Cat#2926; RRID: AB_2070400 |
| Mouse monoclonal anti-Cyclin D3 (DCS22) | Cell Signaling Technology | Cat#2936; RRID: AB_2070801 |
| Rabbit Polyclonal anti-Cyclin E2 | Cell Signaling Technology | Cat#4132; RRID: AB_2071197 |
| Mouse monoclonal anti-BrdU (ZBU30) | Life Technologies | Cat#03–3940; RRID: AB_2532919 |
| Goat IgG | Beyotime Biotechnology | Cat#A7007; |
| Rabbit IgG | Beyotime Biotechnology | Cat#A7016; |
| Rabbit monoclonal anti-CDKN1A | Abclonal | Cat#A1483; RRID: AB_2761709 |
| Anti-rabbit IgG, HRP-linked Antibody | Cell Signaling Technology | Cat#7074; RRID: AB_2099233 |
| Anti-mouse IgG, HRP-linked Antibody | Cell Signaling Technology | Cat#7076; RRID: AB_330924 |
| Bacterial and Virus Strains | ||
| pLKO.1 - TRC cloning vector | (Sarbassov et al., 2005) | Addgene #10878 |
| Scramble control shRNA | (Moffat et al., 2006) | Addgene #1864 |
| Pumi shRNA | This paper | N/A |
| pSin-EF2-Pum1-Pur | This paper | N/A |
| pSin-EF2-Pum1(mutR7)-Pur | This paper | N/A |
| CMV-Pumi | This paper | N/A |
| V14–6 × His-SUMO | Laboratory of Ming Lei | N/A |
| pUC57 | Genewiz | N/A |
| psiCHECK-2 Vector | Promega | Cat#C8021 |
| Chemicals, Reptides, and Recombinant Proteins | ||
| Hartman’s Fixative | Sigma-Aldrich | Cat#H0290 |
| DMEM | GIBCO | Cat#11995073 |
| Fetal Bovine Serum | GIBCO | Cat#10099141 |
| 0.25%Trypsin | Invitrogen | Cat# 25300062 |
| Collagenase | Sigma-Aldrich | Cat# C9891 |
| Deoxyribonuclease I | Sigma-Aldrich | Cat# D4527 |
| BrdU | Roche | Cat# 10280879001 |
| FuGENE HD Transfection Reagent | Rromega | Cat# E2311 |
| PI/RNase Staining Buffer | BD Biosciences | Cat# 550825 |
| Protein A Agarose | Invitrogen | Cat# 15918014 |
| TRIzol Reagent | Invitrogen | Cat# 15596018 |
| RNasin Ribonuclease Inhibitors | Rromega | Cat#N2511 |
| EDTA-free Protease Inhibitor Cocktail | Roche | Cat#1187358001 |
| Cycloheximide | Sigma-Aldrich | Cat# C7698 |
| Criticai Commerciai Assays | ||
| Lenti-Pac HIV Expression Packaging Kit | GeneCopoeia | Cat# HRK-LvTR-20 |
| Click-iT EdU Alexa Fluor® 647 Flow Cytometry Assay Kit | Invitrogen | Cat# C10419 |
| Dual-Luciferase Reporter Assay System | Rromega | Cat# E1910 |
| ClonExpress II One Step Cloning Kit | Vazyme Biotech | Cat# C112–01 |
| In Situ Cell Death Detection Kit | Roche | Cat# 11684817910 |
| FITC Annexin V Apoptosis Detection Kit | BD Biosciences | Cat# 556547 |
| Biotin-Streptavidin HRP Detection Systems | ZSGB-BIO | Cat# SR-9001 |
| LightShift Chemiluminescent RNA EMSA Kit | Thermo scientific | Cat# 20158 |
| Pierce RNA 3′ End Biotinylation Kit | Thermo scientific | Cat# 20160 |
| mMESSAGE mMACHINE® T7 Ultra Kit | Ambion | Cat# AM1345 |
| MEGAclear Transcription Clean-Up Kit | Ambion | Cat#AM1908 |
| PrimeScript RT Reagent Kit | Takara | Cat#RR037A |
| TB Green Premix Ex Taq II | Takara | Cat#RR820A |
| Experimental Models: Cell Lines | ||
| Mouse: MEF | Established by our lab | N/A |
| Mouse: NIH 3T3 | Laboratory of Yan Cheng | N/A |
| Human: 293T | Laboratory of Xin Wu | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Pum1−/−: B6.129 | Estabiished by our lab | N/A |
| Mouse: 129-Cdkn1btm1Mlf/J | The Jackson Laboratory | JAX: 003122 |
| Oligonucleotides | ||
| shRNA targeting sequence: TRCN0000294814: TGA TCTCAAACTGGCATTTAA | This paper | N/A |
| Primers for PCR and RT-qPCR, see EXPERIMENTAL MODEL AND SUBJECT DETAILS | This paper | N/A |
| Software and Algorithms | ||
| FiowJo 7.6.1 | Ashland | https://www.flowjo.com/solutions/flowjo |
| Illustrator CC | Adobe | http://www.adobe.com/products/illustrator.html |
| Photoshop CC | Adobe | http://www.adobe.com/products/photoshop.html |
| MODfit LT 4.0 | Verity Software House | https://www.vsh.com/products/mflt/index.asp |
| GraphPad Prism software 5 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |