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. 2017 May 8;3:17013. doi: 10.1038/micronano.2017.13

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Copyright © 2017 The Author(s)

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Deformability cytometry. (a) Microfluidic device with single inlet and two outlets. Asymmetric focusers and inertial focusing aid in biasing the cells to two vertically stacked equilibrium positions. (b) Cells are delivered uniformly to an extensional flow region where they are deformed. The deformation process is captured using high-speed photography, and parameters associated with size, morphology, strain or deformability, and strain rate are extracted from sequences of images through computer-automated image analysis. (c) Bright-field images of a cell entering the extensional flow and deforming.