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. 2019 Mar 12;10:270. doi: 10.3389/fpls.2019.00270

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Copyright © 2019 Saberianfar, Chin-Fatt, Scott, Henry, Topp and Menassa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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V_HH9-sIgA subunits assemble in vivo over time. N. benthamiana leaves were co-infiltrated with a mixture of the indicated Agrobacterium strains, and leaf samples were collected from 4 to 12 dpi. Extracts were prepared under native conditions and separated with SDS-PAGE under non-reducing conditions. Extract from p19-infiltrated N. benthamiana leaves at 12 dpi was used as negative control. Equal amounts (10 μg) of TSP were loaded per lane. Western blot was detected with anti-HA (A) and anti-c-Myc antibodies (B).