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. 2019 Mar 12;10:270. doi: 10.3389/fpls.2019.00270

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Copyright © 2019 Saberianfar, Chin-Fatt, Scott, Henry, Topp and Menassa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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V_HH10-sIgA binds EHEC O145:Hnm, O26:H11, O111:Hnm, and O157:H7. Shown are confocal images visualizing the binding of the seven most prevalent strains of EHEC with V_HH10-sIgA. Binding is visualized by DAPI that stains EHEC bacterial cells (blue) and a FITC-conjugated antibody that hybridizes to the Fc chain of V_HH10-sIgA (green). A merged image shows an overlay of the blue and green channels used to visualize DAPI and FITC, respectively, to assess co-localization.