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. 2019 Apr 2;9:5515. doi: 10.1038/s41598-019-41941-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Organotypic invasion assay. Representative images of haematoxylin and eosin (H&E) stained sections showing cell lines cultured on top of collagen/fibroblast matrices at day 10 of the organotypic invasion assay. Images captured using Hamamatsu Nanozoomer XR at 40X objective magnification. (A). High-grade serous (HGS) cell lines COV318, FUOV1 and PEO1. (B). Non-serous (NS) cell lines TOV21G, A2780 and SKOV3. TOV21G invaded to a maximum depth of 250 µm. A2780 invaded to a maximum depth of 150 µm. (C). Collagen/fibroblast matrix with no cell lines cultured on top. Experiments were carried out on 3 separate occasions for each cell line.