Figure 3.

Statistical comparison of diversity metrics computed from microbiomes samples grouped by dosage type and study phase. Plot showing results of Mann-Whitney rank-sum test carried out between values obtained for an alpha-diversity measure/metric (e.g. Shannon). For each dosage type (D1, D2, D3, or P), the statistical test was carried out between. (i) set of metric values computed from all samples in the basal phase (B) vs. all samples in the dosage phase (D), (ii) set of metric values computed from all samples in the dosage phase (D) vs. all samples in the follow-up phase (F), and (iii) set of metric values computed from all samples in the basal phase (B) vs. all samples in the follow-up phase (F). For each of the four dosage types (D1, D2, D3, and P), three sets of p-values corresponding to the three types of comparisons viz. (i), (ii), and (iii) mentioned above were obtained for each of the three measures of alpha-diversity (depicted as horizontal panels A–C). Each horizontal panel therefore depicts four subfigures corresponding to results obtained with the four dosage type). In each sub-figure, the p-value obtained for the three comparisons viz. (i), (ii), and (iii) can be visualized and interpreted. For e.g. the top-right square (coloured blue) in the first sub-figure of panel (A) depicts the p-value obtained by statistical comparison between the set of metric values computed from all samples in the dosage phase (D) vs. all samples in the follow-up phase (F). The colours indicated in the legend provided for the figure can be used for interpreting the statistical significance of the p-value that was obtained.