Fig. 2.

Enhanced inflammasome and pathogen receptor signaling in Rnf5^−/− mice. a NanoString analysis of PanCancer Immune Profiling genes in tumors from WT and Rnf5^−/− mice. The heatmap shows 47 genes with >1.2-fold (P < 0.05) differences in expression between YUMM1.5 tumors from the two genotypes (n = 5) at 10 days after injection. b Growth of B16F10 melanoma cells after subcutaneous injection of 10⁶ cells into WT, Rnf5^−/−, MyD88^−/− and MyD88^−/−Rnf5^−/− mice (WT, Rnf5^−/−, n = 6; MyD88^−/−, n = 5; MyD88^−/− Rnf5^−/−, n = 8). c qRT-PCR analysis of pathogen receptor mRNA levels in IECs from tumor-bearing WT or Rnf5^−/− mice (n = 4). d qRT-PCR analysis of cytokine and chemokine mRNA levels in IECs isolated from the small intestines of tumor-bearing WT or Rnf5^−/− mice (n = 4). Data are representative of three independent experiments (c, d), two independent experiments (b) or one experiment (a) ≥5 mice per group. Graphs show the mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.001, ****P < 0.0001 by two-tailed t test or Mann–Whitney U test (c, d) or two-way ANOVA with Sidak’s correction (b)