Table 1.
Overview of all Neo-mAbs generated in this work for the detection of catalytically active BoNT.
| Serotype | Antibodya | Isotype | Specificityb | Endopep-ELISAc |
|---|---|---|---|---|
| BoNT/A | SNAP/A/291 | IgG1 | N | +++ |
| SNAP/A/305 | IgG1 | N | +++ | |
| BoNT/B | VAMP/B/1148 | IgG1 | N | +++ |
| VAMP/B/226 | IgG1 | N | +++ | |
| VAMP/B/151 | IgG2b | C | + | |
| VAMP/B/392 | IgG1 | C | − | |
| VAMP/B/726 | IgG3 | C | − | |
| BoNT/C | SNAP/C/2207 | IgG1 | C | − |
| SNAP/C/5593 | IgG2a | N | +++ | |
| SNAP/C/1844 | IgG2a | N | − | |
| SNAP/C/3280 | IgG2b | N | − | |
| BoNT/D | VAMP/D/27 | IgG2b | C | +++ |
| VAMP/D/29 | IgG2b | C | ++ | |
| BoNT/E | SNAP/E/1466 | IgG1 | C | − |
| SNAP/E/217 | IgG1 | N | + | |
| BoNT/F | VAMP/F/440 | IgG2a | C | +++ |
| VAMP/F/153 | IgG1 | N | − | |
| VAMP/F/521 | IgG1 | N | +++ | |
| VAMP/F/425 | IgG1 | N | +++ | |
| VAMP/F/1333 | IgG1 | N | +++ |
aAntibodies depicted bold were selected for implementation into the functional Luminex suspension array.
bSpecificity of Neo-mAbs towards N- or C-terminal fragment generated after cleavage by indicated BoNT. cEC50 in Endopep-ELISA: + + + = < 0.1 ng/ml; + + = < 1 ng/ml; + = < 10 ng/ml; − = signal too weak for detection.