Table 2.
Assay performance of the duplex-assay for detection of BoNT/A-F substrate cleavage.
| BoNT/A | BoNT/B | BoNT/C | BoNT/D | BoNT/E | BoNT/F | |
|---|---|---|---|---|---|---|
| EC50 (pg/mL) | 70 | 47 | 18901 | 16 | 654c | 17 |
| LOD (pg/mL) | 0.8 | 1.2 | 79.1 | 1.1 | 13 | 0.3 |
| (95% CI)a | (0.6–1.3) | (0.7–5.4) | (45–227) | (0.9–1.3) | (9.2–23.7) | (0.2–0.4) |
| LOD (LD50/mL)b | 0.211 | 0.147 | 2.056 | 0.18d | 0.84 | 0.005 |
aCutoff: mean + 3.29 × SD of blank values (determined with 36 blank values for each serotype) including lower and upper limit of 95% confidence interval (CI; shown in parentheses). bLimit of detection (LOD) in mouse minimal lethal doses (LD50/mL) according to toxin activity as specified by the manufacturer (see Methods). ctrypsinated BoNT/E. dCalculated according to Sugiyama35.