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. 2019 Apr 2;9:5520. doi: 10.1038/s41598-019-42004-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and gene stability of EBOV GP by recombinants in DF-1 cells. (a) and (b) The level of GP expression at 24 h p.i. in DF-1 cells infected with indicated viruses was analyzed by Western blot. Amount of N proteins and GP proteins were quantitated and the ratio of GP to N is shown as bar graph. Results of three independent experiments are shown, and error bars show standard deviation. (c) and (d) The EBOV GP expressing rNDV-3FHN and rAPMV-3 were passaged ten times in DF-1 cells and (e) rNDV-3FHN N/P-GP and rAPMV-3 N/P-GP were passaged twenty times in DF-1 cells. The stability of GP expression was analyzed by Western blot. Amount of N proteins and GP proteins were quantitated and the ratio of GP to N is shown as bar graph. A result of single experiment is shown. *P < 0.05 by one-way ANOVA with Bonferroni post hoc test.