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. 2019 Apr 2;9:5504. doi: 10.1038/s41598-019-41930-7

Figure 2.

Figure 2

Structures of Casp6 rare variants. Crystal structures of mature apo Casp6-E35K (a,b, light pink) and Casp-A109T (c,d, light orange) superimposed on a mature apo structure of Casp6-WT (PDB: 2WDP, grey). The structures are represented as a cartoon with catalytic residues (H121, C163) and mutated residues (E35/K35, A109/T109) shown as sticks. L1, L2, L3, L4, and L2′ depict active site loops. Right panels (b,d) are the zoom-in images of boxed regions in left panels (a,c). The interaction of K35/E35 with R254 and K284 at the dimer interface of apo Casp6-WT crystal structure (e, dark grey LS, light grey SS) and crystal structure of the apo Casp6-E35K (f, light violet LS, light pink SS). Dotted black lines represent weak electrostatic E35 interaction (3.4–3.6 Å distance) with R254 and K284 in Casp6-WT.