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. 2019 Apr 2;9:5483. doi: 10.1038/s41598-019-41476-8

Figure 1.

Figure 1

Skeletal muscle-specific Dicer knockout. (A) Scheme showing the major domains of the Dicer protein with loxP sites flanking exons 23 and 24 which encode RNase IIa and IIb domains, respectively; the RNAse II domain is absolutely required for generation of mature miRNAs. Following tamoxifen administration, Cre-mediated recombination causes excision of exons 23 and 24, thereby promoting Dicer inactivation. (B) PCR analysis using genomic DNA isolated from liver, heart or skeletal muscle shows Cre-mediated recombination (429 bp band) only occurs in skeletal muscle following tamoxifen administration. (C) Dicer mRNA expression was significantly lower (~90%) in skeletal muscle of tamoxifen-treated mice (knockout, KO) compared to vehicle-treated (wild-type, WT) mice. Values are presented as the mean ± SEM (n = 4–6) with expression normalized to WT. Asterisk denotes significant difference between WT and KO groups (p ≤ 0.05).