Table 3.
Usage of primers.
| Primers | Template | Object or construct |
|---|---|---|
| Olit146/Olit176 | first strand cDNA | qPCR for KDM2A mRNA |
| Olit180/Olit169 | first strand cDNA | qPCR for N782 mRNA |
| Olit149/Olit177 | first strand cDNA | qPCR for KRT14 mRNA |
| Olit182/Olit183 | first strand cDNA | qPCR for GAPHD mRNA |
| Olit170/Olit171 | pEGFP-N782 | pET-CXXC |
| Olit172/Olit173 | pBluescript KS(+)− | Amplification of genomic |
| genomic DNA fragments | DNA fragments in the vector | |
| Olit174/Olit181 | KDM2A in pMarXG7 | pEGFP-KDM2A |
| Olit174/Olit175 | KDM2A-N782 in pMarXG7 | pEGFP-N782 |
| Olit184/Olit181 | pEGFP-KDM2A | pEGFP-C387 |
| Olit185/Olit175 | pEGFP-KDM2A | pEGFP-C233 |
| Olit196/Olit197 | pEGFP-N782 | pEGFP-N782 ∆CXXC |
| Olit198/Olit199 | pEGFP-N782 | pEGFP-N782 ∆JmjC |
| Olit200/Olit201 | pGEX-CXXC-PHD-NLSR | pGEX-CN |
| Olit208/Olit209 | pEGFP-N782 | pEGFP-NLSR– > pGEX-NLSR |
| Olit250/Olit209 | pEGFP-N782 | pEGFP-CPN and pGEX-CPN |
| Olit250/Olit209 | pEGFP-N782 ∆PHD | pGEX-CN |
| Olit250/Olit209 | pGEX-CXXC-NLSR | pEGFP-CN |
| Olit253/Olit209 | pEGFP-N782 | pEGFP-PN and pGEX-PN |
| Olit254/Olit255 | First strand cDNAs | pET-XRCC5 |
| Olit256/Olit257 | First strand cDNAs | pET-XRCC6 |
| Olit276/Olit279 | Keratinocyte genomic DNA | pIGFBPL1-687-Gluc–> |
| Olit280/281 | pEGFP-CXXC-NLSR | pCN |
| Olit280/281 | pEGFP-PHD-NLSR | pPN |
| Olit280/281 | pEGFP-CXXC-PHD-NLSR | pCPN |
| Olit280/281 | pEGFP-NLSR | pNLSR |
| Olit280/281 | pEGFP-KDM2A | pKDM2A |
| Olit280/281 | pEGFP-N782 | pN782 |
| Olit288/Olit297 | pIGFBPL1-687-GLuc | IGFBPL1-171 bait |
| Olit288/Olit294 | pIGFBPL1-687-GLuc | IGFBPL1-248 bait |
| Olit288/Olit289 | pIGFBPL1-687-GLuc | IGFBPL1-312 bait |
| Olit288/Olit293 | pIGFBPL1-312 ΔXmal-GLuc | hybrid-312 bait |
| Olit309/Olit310 | pDIP2B-GLuc | DIP2B-312 bait |
| Olit313/Olit293 | pIGFBPL1-312 ΔXmal-GLuc | Vec-243 bait |
| Olit314/Olit315 | pCPN | pMarXG–> pMar-CPN |
| Olit314/Olit315 | pNLSR | pMAXG–> pMar-NLSR |
| Olit318/Olit319 | Keratinocyte genomic DNA | rDNA promoter |