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. 2019 Apr 2;10:1495. doi: 10.1038/s41467-019-09482-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Neat1 enhances the activation of the NLRP3 inflammasome. a–c Control iBMDMs and iBMDMs devoid of Neat1 by shRNA #1 (a, left and b) or #2 (a, right and c) were untreated, primed with LPS, or treated with nigericin after LPS priming. Whole cell lysates (WCL) and the supernatant (SN) and pellet (PE) fractions were analyzed by western blotting for indicated protein levels (a). Culture supernatant was analyzed by ELISA for IL-1β secretion (b, c). d–g iBMDMs devoid of Neat1_2 (d, e) or overexpressing Neat1_1 (f, g) and the corresponding controls were treated with or without LPS or nigericin as indicated, and analyzed for protein levels (d, f) and IL-1β secretion (e, g). h, i BMDMs from Neat1 wild-type (Neat1^+/+) or knockout (Neat1^−/−) mice were primed with LPS and then stimulated with nigericin as indicated. Protein levels (h) and IL-1β secretion (i) were analyzed. In b, c, e, g, and i, data shown are mean ± SD (n = 4). ***P < 0.001, two-tailed t-test. Source data are provided as a Source Data file