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. 2019 Apr 2;10:1495. doi: 10.1038/s41467-019-09482-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — Loss of Neat1 impairs alum-induced peritoneal inflammation and attenuates flagellin-induced lung inflammation in vivo. a IL-1β content in the lavage fluid from Neat1^+/+ or Neat1^−/− mice 12 h after PBS or alum injection. b Absolute numbers of Neat1^+/+ or Neat1^−/− PECs recovered 12 h after PBS or alum injection. c, d Absolute numbers of neutrophils (c) and Ly6C⁺ monocytes (d) exuded to the peritoneum 12 h after PBS or alum injection. e IL-1β content in the BALF from Neat1^+/+ or Neat1^−/− mice 12 h after isotonic saline or flagellin intranasal instillation. f Absolute numbers of Neat1^+/+ or Neat1^−/− PECs recovered 12 h after isotonic saline or flagellin intranasal instillation. g, h Absolute numbers of neutrophils (g) and Ly6C⁺ monocytes (h) exuded to the lung 12 h after isotonic saline or flagellin intranasal instillation. In a–h, data shown are mean ± SD (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test. Source data are provided as a Source Data file