Fig. 3.

The effect of Neat1 on the NLRP3 inflammasome activation is independent of IL-6. a BMDMs derived from IL-6 wild-type (IL-6^+/+) or knockout (IL-6^−/−) mice were primed with LPS and then treated with nigericin. Protein levels in total cell lysates and supernatant fraction were analyzed by western blotting. b–d IL-6^+/+ or IL-6^−/− BMDMs were transfected with control siRNA (b–d), Neat1 siRNA #1 (b), Neat1 siRNA #2 (c), or Neat1_2 siRNA (d). Twenty-four hours later, cells were stimulated with LPS and nigericin. WCL and SN of above cells were subjected to western blotting with indicated antibodies. Source data are provided as a Source Data file