Fig. 5.

Neat1 stabilizes the caspase-1 hetero-tetramers and increases caspase-1 activity. a–d 293T cells were infected with lentiviruses expressing control RNA or Neat1_1 and then co-transfected with Flag-p20 and HA-p10 (a, b) or Flag-p33 and HA-p10 (c, d). Cell lysates were incubated with anti-Flag mAb (M2) beads. Input samples and pull-down products were subjected to western blotting (a, c). Relative ratios of HA-p10 pulled down by Flag-p20 (b) or Flag-p33 (d) were shown in histogram, based on the data of three independent replication experiments. e Neat1^+/+ and Neat1^−/− BMDMs were primed with LPS and then activated with nigericin, flagellin, or poly(dA:dT). Mature caspase-1 activity was measured. f Control or Neat1_1 overexpressing iBMDMs were primed with LPS and then activated with nigericin, flagellin, or poly(dA:dT). Mature caspase-1 activity were measured. In b, d, e and f, data shown are mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test. Source data are provided as a Source Data file