Fig. 6.

Neat1 promotes caspase-1-dependent pyroptosis. a–f Neat1_1-overexpressing iBMDMs (a, b), Neat1-knockdown iBMDMs (c, d), Neat1^−/− BMDMs (e, f), and the corresponding control cells were primed with LPS and then stimulated with nigericin. Cells were stained with PI and analyzed under microscope (a, c, and e). Percentages of pyroptotic cells were measured by LDH release into supernatants (b, d, and f). In a, c, and e, the bar graph indicates 300 μm. In b, d, and f, data shown are mean ± SD (n = 4). ***P < 0.001, two-tailed t-test. Source data are provided as a Source Data file