Figure 3.

(A) Reactivation of recombinant human MAO-B enzyme by irreversible inhibitor selegiline (30 nM), reversible inhibitor safinamide (50 nM), and representative compounds NTZ-1006 (1.0 nM) and NTZ-1091 (1.0 nM). (B) Lineweaver–Burk plot of the inhibition of hMAO-B enzyme in the absence (no inhibitor) and in the presence of different concentrations (0.5 and 1.0 nM) of NTZ-1091. The reciprocal hMAO-B inhibitory activity of the compound was plotted versus the reciprocal substrate concentration. 1/V: 1/velocity of reaction [1/(nmol p-tyramine/min/mg protein)]; 1/[S]: 1/substrate concentration (1/mM p-tyramine).