Table 2.
Opena | Closedb | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cell | Phenotypec | Pt 1 | Pt 2 | Pt 3 | Pt 4 | Pt 5 | Mean ± SEM | Pt 1 | Pt 2 | Pt 3 | Pt 4 | Pt 5 | Mean ± SEM |
Tnaive | CD45RA+/CCR7+ | 3.0 | 9.0 | 1.0 | 7.0 | 60.0 | 16.0 ± 11.1 | 14.0 | 5.0 | 3.0 | 24.0 | 67.0 | 22.6 ± 11.7 |
TCM | CD45RA−/CCR7+ | 37.0 | 20.0 | 22.0 | 31.0 | 1.9 | 22.4 ± 6.0 | 20.0 | 16.0 | 16.0 | 39.0 | 1.0 | 18.4 ± 6.1 |
TEM | CD45RA−/CCR7− | 57.0 | 62.0 | 74.0 | 51.0 | 1.0 | 49.0 ± 12.6 | 35.0 | 67.0 | 75.0 | 27.0 | 1.0 | 41.0 ± 13.5 |
TEMRA | CD45RA+/CCR7− | 3.0 | 9.0 | 3.0 | 12.0 | 37.0 | 12.8 ± 6.3 | 31.0 | 11.0 | 6.0 | 11.0 | 31.0 | 18.0 ± 5.4 |
Open: cells transduced by spinoculation in plates.
Closed: cells transduced in bags using closed production process.
Phenotype shown as percentage of CD3+ lymphocytes.