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. 2019 Mar 28;14:621–630. doi: 10.2147/CIA.S193765

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© 2019 Hai et al. This work is published and licensed by Dove Medical Press Limited

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Melatonin inhibited the effect of miR-106a-5p in AF cells of patients with DD. (A) The expression level of miR-106a-5p was examined in AF cells that transfected with negative control, inhibitor, and treated with M50 or M50 + mimics, respectively. (B) The cell apoptosis profile of AF cells of patients with DD. (C) The relative protein level of LC3-II, ATG7, cleaved caspase-3, and cleaved PARP in AF cells of patients with DD. (D) Representative images of immunofluorescent detection of LC3-II (green) in AF cells of patient with DD.

Notes: *P<0.05 vs control, **P<0.01 vs control, and ***P<0.001 vs control. Magnification of the images is 400×.

Abbreviations: AF, annulus fibrosus; DD, disc degeneration; LC3, light chain 3; M50, melatonin of 50 µmol/L; PARP, poly-ADP-ribose polymerases-1.