SNR-B-mode overlays showing regions of SNR ≥5 dB, ≥9 dB, and ≥13 dB for (A) ≥2 or (B) =5 wavelengths in a representative imaging frame from ex vivo Rat 2. (C) Comparison of the average SO2-estimation error with (green) and without (yellow) LFC when SO2 is calculated with 2, 3, 4, or all 5 wavelengths; an asterisk denotes a significant difference in error compared to the 2-wavelength result.