Figure 3.
Vaginal mucus grade following intrauterine infusion of bacteria. Vaginal mucus was collected using a Metricheck (Metricheck, Simcro, Hamilton, New Zealand) tool and graded according to Sheldon et al. (2009). Vaginal mucus was graded from 0 to 3 and each heifer is represented by a single circle. Control heifers are represented by open circles (A, ○), and bacteria-infused heifers are represented by filled circles (B, ●). The vertical dotted line denotes the day of treatment; solid horizontal lines indicate the median vaginal mucus score for the day of observation. Data were analyzed using the GLIMMIX procedure following a Poisson distribution to determine the effect of treatment (Trt).