FIG. 5.

Histological and immunohistochemical evaluation of neurons, axons, and dendrites in organotypic hippocampal slice cultures (OHSCs) following exposure to single and repetitive Level 2 primary blast. (A-C) Hematoxylin and eosin (H&E) staining of OHSCs receiving the sham (A, scale bar = 1 mm), a single Level 2 blast (B), or two Level 2 blasts 24 h apart (C) revealed intact principal cell body layers. (D-F) SMI-31 staining of OHSCs receiving the sham (D), a single Level 2 blast (E), or two Level 2 blasts 24 h apart (F) showed the majority of axons were undisturbed by primary blast. (G-I) SMI-32 staining of OHSCs receiving the sham (G), a single Level 2 blast (H), or two Level 2 blasts 24 h apart (I) revealed minimal damage to axons. (J-L) Microtubule associated protein 2 (MAP-2) staining of OHSCs receiving the sham (J), a single Level 2 blast (K), or two Level 2 blasts 24 h apart (L) showed the majority of dendrites were undisturbed by primary blast. Color image is available online at www.liebertpub.com/neu