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. 2019 Apr 2;9:5475. doi: 10.1038/s41598-019-41868-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diagram showing the isolation procedure of spontaneous host-range mutants of phage 812 represented by boxes. All phage mutants were isolated from a single plaque on selection S. aureus strains shown next to the corresponding mutants. Phage mutants were then propagated on S. aureus strains that are colour coded according to the phage mutant. The sequence type (ST) and the staphylococcal protein A (spa)-type of each strain are given in parentheses.