Table 4.

Primer pairs used in this study to screen for azoreductase genes.

Bacterial species of interest	Primers			Expected size of amplified DNA fragments (bp)
	Name	Sequence (5′-3′)	Length (bp)
E coli	EcF	GGC GAA GAC AAA TTT YAC ATC RGT AAT G	28	486
	EcR	CAG GGT ACT CTC AGT CTA ATC AGT TGT CC	29
Bacillus cereus	BcF	ACA GCA AAT CCA AAT TCA GCA GAA GG	26	420
	BcR	CTC CTT CAG AAT AWA CGC CAC CTG TT	26
Bacillus subtilis	BsF	GAA TTT TGC AGC HAG ATC TTT TGC TTC TTG	30	471
	BsR	CCT YGG MAG AGA TAT GAT TAA CGG AAC ATT	30
Bacillus thuringiensis	BthF	TTG CTA CTT TAG CTG CTT CTT CAA GAC CHG	30	579
	BthR	CCA GCG GAA CAA GCA GTT AGT GTR AA	26
Enterococcus faecium	EfmF	AGT AGT CAA AGG TCA TCC CCT AAC TGC	27	567
	EfmR	TTT TGC ATC AAC TCA TCT GCA CGG TC	26
Enterococcus faecalis	EfsF	CAA ACA TGC CGG AAA TCG ATG AAG AA	26	311
	EfsR	GAA GCC GCC ATT TGA TTG GAT GT	23

Primer names, sequences, lengths and expected size of amplified DNA fragments (amplicons) are given for each primer. H = A + T + C, M = A + C, R = A + G, W = A + T and Y = C + T.