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. 2019 Apr 2;9:5538. doi: 10.1038/s41598-019-41772-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Unsupervised clustering analysis of the most abundant miRNAs (A) and other small ncRNAs (B). Top 60 most abundant miRNAs and top 40 most abundant other small ncRNAs in any samples were selected for the clustering analysis, resulting in 101 miRNAs and 109 other small ncRNAs. Heatmaps are showing the relative concentration of each RNA, normalized using the spike-in miRNAs. Medium level refers to the concentration range of spike-ins, whereas low level is lower than any of the spike-ins and high level is higher than any of the spike-ins used.