Table 1.
Suppliers and pricing information of culture media used for small ncRNA sequencing.
| Acronym | Culture media | Basal media | Serum manufacturer | Serum content | Supplementation | Price/50 ml (excl. VAT) |
|---|---|---|---|---|---|---|
| FBS-1 | FBS-1 media |
DMEM/F12 + Glutamax cat# 31331093 |
Sigma, ref. 10270106, lot. 42F8554K | 10% FBS | 1% pen-strep | 4 eur |
| FBS-2 | FBS-2 media |
DMEM/F12 + Glutamax cat# 31331093 |
Gibco, ThermoFisher cat# 10270106 lot. 42G8468K |
10% FBS | 1% pen-strep | 4 eur |
| UC-dFBS-1 | Ultracentrifugation EV-depleted FBS media |
DMEM/F12 + Glutamax cat# 31331093 |
Sigma, ref. 10270106, lot. 42F8554K | 10% UC-dFBS | 1% pen-strep | 36 eur |
| UC-dFBS-2 | Ultracentrifugation EV-depleted FBS media |
DMEM/F12 + Glutamax cat# 31331093 |
Gibco, ThermoFisher cat# 10270106 lot. 42G8468K |
10% UC-dFBS | 1% pen-strep | 35 eur |
| UF-dFBS-1 | Ultrafiltration EV-depleted FBS media |
DMEM/F12 + Glutamax cat# 31331093 |
Sigma, ref. 10270106, lot. 42F8554K | 10% UF-dFBS | 1% pen-strep | 53 eur |
| UF-dFBS-2 | Ultrafiltration EV-depleted FBS media |
DMEM/F12 + Glutamax cat# 31331093 |
Gibco, ThermoFisher cat# 10270106 lot. 42G8468K |
10% UF-dFBS | 1% pen-strep | 52 eur |
| SBI-dFBS-1 | Commercial Exosome depleted FBS media |
DMEM/F12 + Glutamax cat# 31331093 |
System Biosciences, ref. EXO-FBS-50A-1 lot.170501-001 | 10% SBI-dFBS | 1% pen-strep | 39 eur |
| SBI-dFBS-2 | Commercial Exosome depleted FBS media |
DMEM/F12 + Glutamax cat# 31331093 |
System Biosciences, ref. EXO-FBS-50A-1 lot.170718-001 | 10% SBI-dFBS | 1% pen-strep | 39 eur |
| StemPRO | Commercial StemPro MSC Serum free medium | StemPro® MSC SFM Basal Medium |
Gibco, Thermo Fisher, A1067501 |
— | 0.3% pen-strep, 1% StemPro MSC CFM Xenofree supplement, GlutaMAX™ Supplement | 37eura |
Pricing information include consumables and supplementation needed for preparation of 50 ml of complete media.
aculture vessels need to be coated with CELLstart™ Substrate (Thermo Fisher cat# A1014201) 1:50 dilution 3.6 eur/ml.