Figure 6. Reductions in hindlimb muscle mass and histological comparison of PD21 rectus femoris.

Representative medial (A) and lateral (B) views of whole hindlimb musculature show an overall reduction in muscle mass in PD21 male Ahi1^−/− mice. Labeling of Na⁺/K⁺ ATPase α1 (green) was used to identify sarcolemma in cross section of RF in male Ahi1^+/+ (C) and Ahi1^−/− (D) mice at PD21, revealing reduced CSA in Ahi1^−/− mice (E). Quantification of nuclei also revealed reduced nuclei per muscle fiber (F) and smaller cross sectional myonuclear domain in Ahi1^−/− mice (* = P<0.05, ** = P<0.01, Student’s t-test; n = 3 per group). Scale bar = 5 mm (A, B); 25 μm (C, D). Data are presented as the mean ± standard error of the mean (SEM).