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ESI mass spectra of mixtures of AT (1.8 μM) and fXa (1.5 μM) incubated in the absence of hepain oligomers (bottom) and in the presence of ca. 15 μM short-chain oligomers (as indicated on each panel) in 150 mM ammonium acetate (pH 7.0). Note that the detectable fXa signal (m/z region 3,000 – 3,700) can be observed only in the absence of heparin oligomers.
