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. 2019 Apr 1;2(2):e201800195. doi: 10.26508/lsa.201800195

Figure 3. NK cells show a mature phenotype and cytotoxicity in NSG hL-7xhIL-15 humanized mice.

Figure 3.

(A) Representative flow cytometry plots of splenic NK cell maturation in conventional NSG and hIL-7xhIL-15 KI NSG humanized mice (IL7xIL15). (B) Increased frequencies of CD56+CD94+CD16+ mature NK cells are detected in the BM, spleen, and PB of hIL-7xhIL-15 KI NSG humanized mice (NSG: BM and spleen n = 20, PB n = 17; IL7xIL15: BM and spleen n = 16, PB n = 13). (C) Representative flow cytometry plots of cytoplasmic expression of granzyme B and perforin in the spleen of NSG and hIL-7xhIL-15 KI NSG humanized mice. (D) Frequencies of granzyme B– and perforin-positive cells among splenic CD56+ NK cells in NSG (n = 13) and hIL-7xhIL-15 KI NSG (n = 10) humanized mice. (E) In vitro cytotoxicity of human NK cells isolated from spleen of conventional NSG, hIL-7xhIL-15 KI NSG humanized mouse spleen (circles) and BM (triangles), and from normal human PB samples (squares) against K562 are shown (NSG n = 3; IL7xIL15 n = 7; normal human PB n = 2). Error bars represent mean ± SEM. *P < 0.05, **P < 0.001, by two-tailed t test.