Table 1.
Engraftment of human leukocytes in NSG mice expressing hIL-7 and/or hIL-15.
| Mouse ID | CB ID | Strain | Weeka | Spleen, % | PB, % | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| hCD45+b | CD56+ | CD3+ | CD19+ | CD33+ | hCD45+b | CD56+ | CD3+ | CD19+ | CD33+ | ||||
| IL7x15#1 | CB#1 | hIL-7xhIL-15 KI | 15 | 46.6 | 35.3 | 11.4 | 19.2 | 15.3 | 15.0 | 60.4 | 6.5 | 2.4 | 6.1 |
| IL7x15#2 | CB#1 | hIL-7xhIL-15 KI | 18 | 35.8 | 38.6 | 1.3 | 11.1 | 10.4 | 30.5 | 68.5 | 1.3 | 11.1 | 10.4 |
| IL7x15#3 | CB#2 | hIL-7xhIL-15 KI | 10 | 98.1 | 13.0 | 0.1 | 78.8 | 3.1 | 93.6 | 38.5 | 0.3 | 42.2 | 7.8 |
| IL7x15#4 | CB#2 | hIL-7xhIL-15 KI | 16 | 92.7 | 3.8 | 23.5 | 57.8 | 5.7 | 95.9 | 19.2 | 37.4 | 15.4 | 10.1 |
| IL7x15#5 | CB#3 | hIL-7xhIL-15 KI | 13 | 76.0 | 18.7 | 0.2 | 67.1 | 3.5 | 22.3 | 70.6 | 0.1 | 11.7 | 9.4 |
| IL7x15#6 | CB#4 | hIL-7xhIL-15 KI | 19 | 77.7 | 22.1 | 2.5 | 49.0 | 4.1 | 30.4 | 37.7 | 2.5 | 26.7 | 4.9 |
| IL7x15#7 | CB#4 | hIL-7xhIL-15 KI | 22 | 66.8 | 26.0 | 26.8 | 26.5 | 3.6 | 18.8 | 38.3 | 33.1 | 21.0 | 1.4 |
| IL7x15#8 | CB#4 | hIL-7xhIL-15 KI | 23 | 64.4 | 16.2 | 10.1 | 16.2 | 6.6 | 15.7 | 6.0 | 18.2 | 24.3 | 3.1 |
| IL7x15#9 | CB#5 | hIL-7xhIL-15 KI | 13 | 80.6 | 36.6 | 1.7 | 40.8 | 8.2 | 49.2 | 47.2 | 1.2 | 19.3 | 20.0 |
| IL7x15#10 | CB#6 | hIL-7xhIL-15 KI | 19 | 65.9 | 52.9 | 1.0 | 17.3 | 14.5 | 30.8 | 64.9 | 0.6 | 6.9 | 8.3 |
| IL7x15#11 | CB#7 | hIL-7xhIL-15 KI | 16 | 97.0 | 1.2 | 74.6 | 15.4 | 3.4 | 98.7 | 9.8 | 69.2 | 1.2 | 1.0 |
| IL7x15#12 | CB#7 | hIL-7xhIL-15 KI | 17 | 92.3 | 2.6 | 72.5 | 17.6 | 2.6 | 85.8 | 14.3 | 67.6 | 3.6 | 1.8 |
| IL7x15#13 | CB#8 | hIL-7xhIL-15 KI | 13 | 51.1 | 21.7 | 0.9 | 60.0 | 11.5 | 4.8 | 37.6 | 0.7 | 13.3 | 22.6 |
| IL7x15#14 | CB#9 | hIL-7xhIL-15 KI | 20 | 28.8 | 56.0 | 2.5 | 11.5 | 9.2 | 11.6 | 70.2 | 1.4 | 7.6 | 10.5 |
| IL7x15#15 | CB#10 | hIL-7xhIL-15 KI | 11 | 78.7 | 9.4 | 0.4 | 76.1 | 5.1 | 77.1 | 25.2 | 0.3 | 34.7 | 27.9 |
| IL7x15#16 | CB#11 | hIL-7xhIL-15 KI | 15 | 58.7 | 72.8 | 1.1 | 12.9 | 6.7 | 7.4 | 68.8 | 0.0 | 8.5 | 15.3 |
| IL7x15#17 | CB#12 | hIL-7xhIL-15 KI | 15 | 74.1 | 54.1 | 0.8 | 27.8 | 8.1 | 20.1 | 55.4 | 0.9 | 20.8 | 2.0 |
| IL7x15#18 | CB#13 | hIL-7xhIL-15 KI | 8 | 68.9 | 25.6 | 0.0 | 54.1 | 7.8 | 7.7 | 24.6 | 0.0 | 29.1 | 33.0 |
| IL7x15#19 | CB#14 | hIL-7xhIL-15 KI | 16 | 23.9 | 87.5 | 0.5 | 7.6 | 1.5 | 2.2 | 82.8 | 0.8 | 5.5 | 3.1 |
| IL7x15#20 | CB#15 | hIL-7xhIL-15 KI | 9 | 56.9 | 31.3 | 0.0 | 32.3 | 22.5 | 35.9 | 60.0 | 0.1 | 8.2 | 18.2 |
| IL7x15#21 | CB#16 | hIL-7xhIL-15 KI | 11 | 46.5 | 61.5 | 1.2 | 24.1 | 3.6 | 31.2 | 70.7 | 1.2 | 12.4 | 4.9 |
| IL7x15#22 | CB#17 | hIL-7xhIL-15 KI | 10 | 90.4 | 8.6 | 0.4 | 72.5 | 8.6 | 75.6 | 29.8 | 0.8 | 35.6 | 28.3 |
| IL7x15#23 | CB#17 | hIL-7xhIL-15 KI | 11 | 77.3 | 7.7 | 0.5 | 77.3 | 10.1 | 61.7 | 13.8 | 0.8 | 49.2 | 27.5 |
| IL7KI#1 | CB#13 | hIL-7 KI | 18 | 99.5 | 0.4 | 1.5 | 11.5 | 83.9 | 97.2 | 1.1 | 4.4 | 4.6 | 85.4 |
| IL7KI#2 | CB#13 | hIL-7 KI | 19 | 87.9 | 5.0 | 10.7 | 36.6 | 37.9 | 83.3 | 11.7 | 20.0 | 23.4 | 27.8 |
| IL7KI#3 | CB#14 | hIL-7 KI | 21 | 90.5 | 3.1 | 10.3 | 25.9 | 57.1 | 59.9 | 4.5 | 18.4 | 40.9 | 25.6 |
| IL7KI#4 | CB#14 | hIL-7 KI | 19 | 93.7 | 1.9 | 10.4 | 49.4 | 34.5 | 98.4 | 1.9 | 63.4 | 14.1 | 15.0 |
| IL7TG#5 | CB#15 | hIL-7 Tg | 16 | 97.8 | 1.2 | 8.0 | 58.6 | 22.2 | 92.3 | 1.7 | 32.1 | 25.2 | 26.2 |
| IL7TG#6 | CB#16 | hIL-7 Tg | 12 | 81.0 | 0.6 | 2.5 | 73.9 | 8.5 | 93.1 | 0.7 | 2.2 | 73.9 | 14.4 |
| IL7TG#7 | CB#17 | hIL-7 Tg | 24 | 95.6 | 0.3 | 2.1 | 11.3 | 84.9 | 81.9 | 0.3 | 0.2 | 2.8 | 95.4 |
| NSG#1 | CB#1 | NSG | 20 | 79.8 | 0.3 | 48.6 | 40.1 | 1.6 | 43.6 | 0.2 | 91.1 | 5.0 | 0.7 |
| NSG#2 | CB#2 | NSG | 17 | 78.1 | 0.2 | 77.5 | 19.1 | 1.4 | 68.5 | 0.2 | 96.9 | 1.0 | 0.2 |
| NSG#3 | CB#2 | NSG | 18 | 85.9 | 0.6 | 14.8 | 74.5 | 3.9 | 37.8 | 0.6 | 50.8 | 28.9 | 11.4 |
| NSG#4 | CB#3 | NSG | 18 | 90.3 | 1.2 | 7.5 | 76.7 | 6.4 | 59.8 | 1.2 | 15.2 | 51.6 | 17.2 |
| NSG#5 | CB#3 | NSG | 19 | 92.4 | 1.2 | 20.5 | 72.4 | 3.5 | 81.5 | 2.5 | 44.0 | 44.3 | 3.6 |
| NSG#6 | CB#3 | NSG | 20 | 91.7 | 1.1 | 10.8 | 72.6 | 5.4 | 68.1 | 1.0 | 28.2 | 36.7 | 22.4 |
| NSG#7 | CB#5 | NSG | 18 | 71.6 | 0.9 | 52.4 | 47.9 | 1.2 | 37.4 | 0.8 | 74.5 | 20.5 | 1.7 |
| NSG#8 | CB#5 | NSG | 19 | 90.2 | 0.2 | 77.6 | 24.0 | 0.9 | 79.9 | 0.6 | 89.2 | 7.0 | 2.3 |
| NSG#9 | CB#7 | NSG | 17 | 98.8 | 0.1 | 64.8 | 30.9 | 1.2 | 97.9 | 0.6 | 83.3 | 10.6 | 0.3 |
| NSG#10 | CB#8 | NSG | 19 | 88.6 | 2.3 | 40.9 | 48.2 | 6.4 | 36.1 | 1.9 | 46.1 | 42.9 | 4.9 |
| NSG#11 | CB#8 | NSG | 19 | 88.5 | 0.7 | 35.3 | 58.1 | 4.8 | 39.4 | 0.3 | 46.6 | 48.7 | 2.3 |
| NSG#12 | CB#10 | NSG | 20 | 98.6 | 0.1 | 60.2 | 32.1 | 0.8 | 97.0 | 0.5 | 85.5 | 7.7 | 1.3 |
| NSG#13 | CB#10 | NSG | 20 | 93.9 | 0.8 | 56.7 | 38.7 | 3.2 | 79.8 | 2.3 | 78.8 | 7.2 | 5.6 |
| NSG#14 | CB#12 | NSG | 19 | 84.4 | 1.0 | 37.8 | 55.8 | 3.3 | 56.7 | 0.9 | 54.1 | 41.7 | 1.6 |
| NSG#15 | CB#12 | NSG | 21 | 85.1 | 0.7 | 40.4 | 53.1 | 2.7 | 49.9 | 0.7 | 56.0 | 34.7 | 3.8 |
| NSG#16 | CB#13 | NSG | 22 | 95.9 | 0.2 | 75.9 | 13.4 | 2.2 | 97.4 | 0.4 | 90.0 | 2.2 | 3.6 |
| NSG#17 | CB#13 | NSG | 23 | 78.5 | 1.8 | 61.8 | 27.1 | 3.9 | 28.5 | 4.3 | 64.8 | 18.9 | 3.5 |
| NSG#18 | CB#14 | NSG | 21 | 82.1 | 0.4 | 38.8 | 56.4 | 2.3 | 31.5 | 0.2 | 63.3 | 29.1 | 3.4 |
| NSG#19 | CB#18 | NSG | 17 | 93.7 | 0.7 | 55.3 | 38.9 | 1.5 | 62.0 | 1.0 | 75.8 | 18.5 | 1.8 |
| NSG#20 | CB#18 | NSG | 19 | 83.3 | 0.6 | 49.7 | 43.9 | 0.9 | 66.1 | 0.6 | 88.9 | 6.3 | 0.1 |
| NSG#21 | CB#19 | NSG | 20 | 99.7 | 0.3 | 83.0 | 13.4 | 1.4 | 99.0 | 0.5 | 90.9 | 4.9 | 1.5 |
| NSG#22 | CB#18 | NSG | 19 | 89.9 | 1.7 | 54.5 | 36.5 | 2.1 | 64.7 | 1.4 | 76.5 | 18.1 | 1.4 |
| NSG#23 | CB#18 | NSG | 19 | 93.6 | 0.5 | 16.0 | 79.6 | 1.0 | 81.7 | 0.5 | 20.2 | 76.7 | 0.2 |
| NSG#24 | CB#19 | NSG | 17 | 87.0 | 2.0 | 46.4 | 45.1 | 3.3 | 42.8 | 7.0 | 65.9 | 14.1 | 2.8 |
a
Number of weeks after CB transplantation when euthanized are shown.
b
Frequencies of human CD45+ cells in total leukocytes including human and mouse CD45+ cells.