Table 1.
1H chemical shift (δ in ppm) and coupling constants (J in Hz) of some metabolites detected in the transgenic and control cell cultures of Catharanthus roseus
| Compounds | Chemical shift (ppm) and coupling constant (Hz) |
|---|---|
| Leucine | δ 0.97 (d, J = 6.8); δ 0.99 (d, J = 6.8) |
| Isoleucine | δ 0.96 (t, J = 7.5); δ 1.03 (d, J = 7.0) |
| Valine | δ 1.01 (d, J = 7.0); δ 1.06 (d, J = 7.0) |
| Threonine | δ 1.34 (d, J = 6.6) |
| Alanine | δ 1.49 (d, J = 7.2) |
| Glutamic acid | δ 2.04 (m); δ 2.12 (m); δ 2.39 (m) |
| Glutamine | δ 2.13 (m); δ 2.46 (m) |
| Malic acid | δ 2.68 (dd, J = 15.4, 3.3); δ 4.28 (dd, J = 9.5, 3.2) |
| Aspartic acid | δ 2.82 (dd, J = 17.0, 8.0); δ 2.95 (dd, J = 16.8, 4.0); δ 3.92 (dd, J = 8.4, 4.0) |
| Sucrose | δ 4.18 (d, J = 8.6); δ 5.41 (d, J = 3.8) |
| Glucose | δ 4.58 (d, J = 8.0, β-form); δ 5.19 (d, J = 3.8, α-form) |
| Fumaric acid | δ 6.52 (s) |
| Tyrosine | δ 6.85 (d, J = 8.5) δ 7.19 (d, J = 8.5) |
| Tryptophan | δ 7.14 (t, J = 7.5); δ 7.22 (t, J = 7.5); δ 7.29 (s); δ 7.48 (d, J = 8.0); δ 7.73 (d, J = 8.0) |
| Tryptamine | δ 7.14 (t, J = 7.5); δ 7.22 (t, J = 7.5); δ 7.28 (s); δ 7.48 (d, J = 8.0); δ 7.65 (d, J = 8.0) |
| Phenylalanine | δ 7.36 (m) |
| Formic acid | δ 8.48 (s) |
s singlet, d doublet, dd double doublet, t triplet, m multiplet