Table 2. Oligonucleotides used in this study.
| Oligo- nucleotide |
Sequence (5’ to 3’) | Description | Refer-ence |
|---|---|---|---|
| pBBE22-bad16usF | GGATAGCATAGAGGTACCCGGGGATCCCAAACCTAAATATGGTCTTAAAGTAAAGATAG | Oligonucleotide pair used to amplify 1639 bp containing bad16 and the upstream/down region from B. afzelii PGau with a 27 bp 5' and a 22 bp 3' flanking region homologous to pBBE22luc vector, including the BamHI site SalI sites. | This study |
| bad16ds- pBBE22R |
GCTTGCATGCCTGCAGGTCGACCATATTCTGATATATCCTGTAAACAGTGTT | ||
| pBBE22- bgd19usF |
GGATAGCATAGAGGTACCCGGGGATCCTTAGCAGCAACTGAAAAATTAGACAAAGC |
Oligonucleotide pair used to amplify 1733 bp containing bgd19 and the upstream/down region from B. garinii IP90 with a 27 bp 5' and a 22 bp 3' flanking region homologous to pBBE22luc vector, including the BamHI site SalI sites. | This study |
| bgd19ds- pBBE22R |
GCTTGCATGCCTGCAGGTCGACAATTCTGATATAGCTTAAACAATATTTTTGAC | ||
| pncAf | TATTGGAATTAATAGGCGGTGATG | Oligonucleotide pair used to confirm pBAD16, pBGD19, and pCD100 constructs | This study |
| lucf | GAGGGGTTGTATTTGTTGACG | ||
| qRT- bad16F |
TGGTGAAAGTGGTGAATTGAAGG | Oligonucleotide pair used in the qRT-PCR to check for bad16 expression in B314/pBAD16. | This study |
| qRT- bad16R |
AGAATTTGAGCCTGAAATAGCTTG | ||
| qRT-bgd19F | TTCCCTTAGCGGTGAAAGTGGTG | Oligonucleotide pair used in the qRT-PCR to check for bgd19 expression in B314/pBGD19. | This study |
| qRT-bgd19R | CTTGATCCTGAAATGCCTTGTAGG | ||
| flaBf | CAGCTAATGTTGCAAATCTTTTCTCT | Oligonucleotide pair used in the qRT-PCR to check for flaB expression in all the B314 background strains. | Hyde et al., 2007 |
| flaBr | TTCCTGTTGAACACCCTCTTGA | ||
| BBK32f | GAATATAAAGGGATGACTCAAGGAAGTT | Oligonucleotide pair used in the qRT-PCR to check for bbk32 expression in B314/pCD100. | Hyde et al., 2007 |
| BBK32r | TTTGGCCTTAAATCAGAATCTATAGTAAGA |